- [Selected Papers for Clock, Sleep and Drosophila Research]

- Tomita J, Ueno T, Mitsuyoshi M, Kume S, Kume K*.
The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster.
PLoS One 10, e0128101. (2015).

- Ueno T, Kume K*: Functional characterization of dopamine transporter in vivo using Drosophila melanogaster behavioral assays. Front Behav Neurosci 8: 303. (2014).

- Ueno T, Tomita J, Tanimoto H, Endo K, Ito K, Kume S, Kume K*.
Identification of a dopamine pathway that regulates sleep and arousal in Drosophila.
Nat Neurosci 15, 1516-1523. (2012).

- Ueno T, Tomita J, Kume S, Kume K*.
Dopamine modulates metabolic rate and temperature sensitivity in Drosophila melanogaster.
PLoS One 7, e31513. (2012).

- Ueno T, Masuda N, Kume S, Kume K*.
Dopamine modulates the rest period length without perturbation of its power law distribution in Drosophila melanogaster.
PLoS One 7, e32007. (2012).

- Takahama K, Tomita J, Ueno T, Yamazaki M, Kume S, Kume K.
Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila.
Biochem Biophys Res Communic 417, 807-11. (2012).

- Yamazaki M, Tomita J, Takahama K, Ueno T, Mitsuyoshi M, Sakamoto E, Kume S, Kume K.
High calorie diet augments age-associated sleep impairment in Drosophila.
Biochem Biophys Res Communic 417, 812-6. (2012).

- Tomita J, Mitsuyoshi M, Ueno T, Aso Y, Tanimoto H, Nakai Y, Aigaki T, Kume S, Kume K.
Pan-Neuronal Knockdown of Calcineurin Reduces Sleep in the Fruit Fly, Drosophila Melanogaster.
J Neurosci 31, 13137-46. (2011).

- Kawabata M, Ueno T, Tomita J, Kawatani J, Tomoda A, Kume S, Kume K.
Temporal organization of rest defined by actigraphy data in healthy and childhood chronic fatigue syndrome children.
BMC Psych 13, 281. (2013).

- Ueno T, Fukuhara A, Ikegami A, Ohishi F, Kume K.
Monozygotic twins concordant for Kleine-Levin syndrome.
BMC Neurol 12, 31. (2012).

- Kume K, Kume S, Park SK, Hirsh J, Jackson FR.
Dopamine is a regulator of arousal in the fruit fly.
J Neurosci 25, 7377-84. (2005).

- Kume K, Zylka MJ, Sriram S, Shearman LP, Weaver DR, Jin X, Maywood ES, Hastings MH, Reppert SM.
mCRY1 and mCRY2 are essential components of the negative limb of the circadian clock feedback loop. (COVER).
Cell 98, 193-205. (1999).

- Shearman LP, Sriram S, Weaver DR, Maywood ES, Chaves I, Zheng B, Kume K, Lee CC, van der Horst GT, Hastings MH, Reppert SM.
Interacting molecular loops in the mammalian circadian clock. (COVER).
Science 288, 1013-9. (2000).

---

- [Selected Papers of other fields and collaborations]

- Sakano D, Shiraki N, Kikawa K, Yamazoe T, Kataoka M, Umeda K, Araki K, Mao, Di, Matsumoto S, Nakagata N, Andersson O, Stainier D, Endo F, Kume K, Uesugi M, Kume S. VMAT2 Identified as a Regulator of Late-Stage β-Cell Differentiation. Nat Chem Biol 10, 141-8. (2014).

- Ida T, Takahashi T, Tominaga H, Sato T, Sano H, Kume K, Ozaki M, Hiraguchi T, Shiotani H, Terajima S, Nakamura Y, Mori K, Yoshida M, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M. Isolation of the bioactive peptides CCHamide-1 and CCHamide-2 from Drosophila and their putative role in appetite regulation as ligands for G protein-coupled receptors. Front Endocrinol 3, 177. (2012).

- Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells 26, 874-85. (2008).

- Riemensperger T, Isabel G, Coulom H, Neuser K, Seugnet L, Kume K, Iché-Torres M, Cassar M, Strauss R, Preat T, et al. Behavioral consequences of dopamine deficiency in the Drosophila central nervous system. Proc Natl Acad Sci USA 108, 834-9. (2011).

- Nakai Y, Horiuchi J, Tsuda M, Takeo S, Akahori S, Matsuo T, Kume K, Aigaki T. Calcineurin and Its Regulator Sra/DSCR1 Are Essential for Sleep in Drosophila. J Neurosci 31, 12759-66. (2011).

- Morales J, Hiesinger PR, Schroeder AJ, Kume K, Verstreken P, Jackson FR, Nelson DL, Hassan BA. Drosophila fragile X protein, DFXR, regulates neuronal morphology and function in the brain. Neuron 34, 961-72. (2002).

- Hendricks JC, Lu S, Kume K, Yin JCP, Yang Z, Sehgal A. Gender dimorphism in the role of cycle (BMAL1) in rest, rest regulation, and longevity in Drosophila melanogaster. J Biol Rhythms 18, 12-25. (2003).

- Nagase T, Uozumi N, Ishii S, Kume K, Izumi T, Ouchi Y, Shimizu T. Acute lung injury by sepsis and acid aspiration: a key role for cytosolic phospholipase A2. Nat Immunol 1, 42-6. (2000).

- Kume K, Shimizu T, Seyama Y. Characterization of sn-glycerol 3-phosphate acyltransferase from guinea pig harderian gland microsomes. J Biochem 101, 653-60. (1987).

- Okazaki K, Okazaki N, Kume K, Jinno S, Tanaka K, Okayama H. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res 18, 6485-9. (1990).

- Kume K, Jinno S, Miwatani H, Kizaka-Kondoh S, Terada Y, Nojima H, Okayama H. Oncogenic signal-induced ability to enter S phase in the absence of anchorage is the mechanism for the growth of transformed NRK cells in soft agar. New Biol 4, 504-11. (1992).

- Uozumi N, Kume K, Nagase T, Nakatani N, Ishii S, Tashiro F, Komagata Y, Maki K, Ikuta K, Ouchi Y, et al. Role of cytosolic phospholipase A2 in allergic response and parturition. Nature 390, 618-22. (1997).

- Ishii S, Nagase T, Tashiro F, Ikuta K, Sato S, Waga I, Kume K, Miyazaki J, Shimizu T. Bronchial hyperreactivity, increased endotoxin lethality and melanocytic tumorigenesis in transgenic mice overexpressing platelet-activating factor receptor. EMBO J 16, 133-42. (1997).

- Kume K, Shimizu T. Platelet-activating factor (PAF) induces growth stimulation, inhibition, and suppression of oncogenic transformation in NRK cells overexpressing the PAF receptor. J Biol Chem 272, 22898-904. (1997).

- Ito K, Suzuki H, Hirohashi T, Kume K, Shimizu T, Sugiyama Y. Molecular cloning of canalicular multispecific organic anion transporter defective in EHBR. Am J Physiol 272, G16-22. (1997).

- Kume K, Shimizu T. cDNA cloning and expression of murine 1-acyl-sn-glycerol-3-phosphate acyltransferase. Biochem Biophys Res Commun 237, 663-6. (1997).

- Kume K, Waga I, Shimizu T. Microplate chromatography assay for acetyl-CoA: lysoplatelet-activating factor acetyltransferase. Anal Biochem 246, 118-22. (1997).

- Ishii S, Kuwaki T, Nagase T, Maki K, Tashiro F, Sunaga S, Cao WH, Kume K, Fukuchi Y, Ikuta K, et al. Impaired anaphylactic responses with intact sensitivity to endotoxin in mice lacking a platelet-activating factor receptor. J Exp Med 187, 1779-88. (1998).

- Nagase T, Ishii S, Kume K, Uozumi N, Izumi T, Ouchi Y, Shimizu T. Platelet-activating factor mediates acid-induced lung injury in genetically engineered mice. J Clin Invest 104, 1071-6. (1999).

- [All Original Articles - Kazuhiko Kume 1987~2015]

1. Kume K, Shimizu T, Seyama Y. Characterization of sn-glycerol 3-phosphate acyltransferase from guinea pig harderian gland microsomes. J Biochem 101, 653-60. (1987).

2. Okazaki K, Okazaki N, Kume K, Jinno S, Tanaka K, Okayama H. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res 18, 6485-9. (1990).

3. Kume K, Jinno S, Miwatani H, Kizaka-Kondoh S, Terada Y, Nojima H, Okayama H. Oncogenic signal-induced ability to enter S phase in the absence of anchorage is the mechanism for the growth of transformed NRK cells in soft agar. New Biol 4, 504-11. (1992).

4. Ferby IM, Waga I, Sakanaka C, Kume K, Shimizu T. Wortmannin inhibits mitogen-activated protein kinase activation induced by platelet-activating factor in guinea pig neutrophils. J Biol Chem 269, 30485-8. (1994).

5. Mutoh H, Kume K, Sato S, Kato S, Shimizu T. Positive and negative regulations of human platelet-activating factor receptor transcript 2 (tissue-type) by estrogen and TGF-beta 1. Biochem Biophys Res Commun 205, 1130-6. (1994).

6. Ferby IM, Waga I, Hoshino M, Kume K, Shimizu T. Wortmannin inhibits mitogen-activated protein kinase activation by platelet-activating factor through a mechanism independent of p85/p110-type phosphatidylinositol 3-kinase. J Biol Chem 271, 11684-8. (1996).

7. Mori M, Aihara M, Kume K, Hamanoue M, Kohsaka S, Shimizu T. Predominant expression of platelet-activating factor receptor in the rat brain microglia. J Neurosci 16, 3590-600. (1996).

8. Ito K, Suzuki H, Hirohashi T, Kume K, Shimizu T, Sugiyama Y. Expression of a putative ATP-binding cassette region, homologous to that in multidrug resistance-associated protein (MRP), is hereditarily defective in Eisai hyperbilirubinemic rats (EHBR). Int Hepatol Commun 4, 291-298. (1996).

9. Yokomizo T, Ogawa Y, Uozumi N, Kume K, Izumi T, Shimizu T. cDNA cloning, expression, and mutagenesis study of leukotriene B4 12-hydroxydehydrogenase. J Biol Chem 271, 2844-50. (1996).

10. Ishii S, Matsuda Y, Nakamura M, Waga I, Kume K, Izumi T, Shimizu T. A murine platelet-activating factor receptor gene: cloning, chromosomal localization and up-regulation of expression by lipopolysaccharide in peritoneal resident macrophages. Biochem J 314, 671-8. (1996).

11. Waga I, Kume K, Ferby I, Honda Z, Shimizu T. Micro-trap phosphorylation assay of mitogen-activated protein (MAP) kinases to detect their activation by lipopolysaccharides. J Immunol Methods 190, 71-7. (1996).

12. Uozumi N, Kume K, Nagase T, Nakatani N, Ishii S, Tashiro F, Komagata Y, Maki K, Ikuta K, Ouchi Y, et al. Role of cytosolic phospholipase A2 in allergic response and parturition. Nature 390, 618-22. (1997).

13. Ishii S, Nagase T, Tashiro F, Ikuta K, Sato S, Waga I, Kume K, Miyazaki J, Shimizu T. Bronchial hyperreactivity, increased endotoxin lethality and melanocytic tumorigenesis in transgenic mice overexpressing platelet-activating factor receptor. EMBO J 16, 133-42. (1997).

14. Tabuchi S, Kume K, Aihara M, Ishii S, Mishina M, Shimizu T. Lipid mediators modulate NMDA receptor currents in a Xenopus oocyte expression system. Neurosci Lett 237, 13-6. (1997).

15. Kume K, Shimizu T. Platelet-activating factor (PAF) induces growth stimulation, inhibition, and suppression of oncogenic transformation in NRK cells overexpressing the PAF receptor. J Biol Chem 272, 22898-904. (1997).

16. Ito K, Suzuki H, Hirohashi T, Kume K, Shimizu T, Sugiyama Y. Molecular cloning of canalicular multispecific organic anion transporter defective in EHBR. Am J Physiol 272, G16-22. (1997).

17. Kume K, Shimizu T. cDNA cloning and expression of murine 1-acyl-sn-glycerol-3-phosphate acyltransferase. Biochem Biophys Res Commun 237, 663-6. (1997).

18. Kume K, Waga I, Shimizu T. Microplate chromatography assay for acetyl-CoA: lysoplatelet-activating factor acetyltransferase. Anal Biochem 246, 118-22. (1997).

19. Ito K, Suzuki H, Hirohashi T, Kume K, Shimizu T, Sugiyama Y. Functional analysis of a canalicular multispecific organic anion transporter cloned from rat liver. J Biol Chem 273, 1684-8. (1998).

20. Hirohashi T, Suzuki H, Ito K, Ogawa K, Kume K, Shimizu T, Sugiyama Y. Hepatic expression of multidrug resistance-associated protein-like proteins maintained in eisai hyperbilirubinemic rats. Mol Pharmacol 53, 1068-75. (1998).

21. Shimada A, Ota Y, Sugiyama Y, Sato S, Kume K, Shimizu T, Inoue S. In situ expression of platelet-activating factor (PAF)-receptor gene in rat skin and effects of PAF on proliferation and differentiation of cultured human keratinocytes. J Invest Dermatol 110, 889-93. (1998).

22. Hirabayashi T, Kume K, Shimizu T. Conditional expression of the dual-specificity phosphatase PYST1/MKP-3 inhibits phosphorylation of cytosolic phospholipase A2 in Chinese hamster ovary cells. Biochem Biophys Res Commun 253, 485-8. (1998).

23. Kinoshita S, Suzuki H, Ito K, Kume K, Shimizu T, Sugiyama Y. Transfected rat cMOAT is functionally expressed on the apical membrane in Madin-Darby canine kidney (MDCK) cells. Pharm Res 15, 1851-6. (1998).

24. Ishii S, Kuwaki T, Nagase T, Maki K, Tashiro F, Sunaga S, Cao WH, Kume K, Fukuchi Y, Ikuta K, et al. Impaired anaphylactic responses with intact sensitivity to endotoxin in mice lacking a platelet-activating factor receptor. J Exp Med 187, 1779-88. (1998).

25. Nagase T, Ishii S, Kume K, Uozumi N, Izumi T, Ouchi Y, Shimizu T. Platelet-activating factor mediates acid-induced lung injury in genetically engineered mice. J Clin Invest 104, 1071-6. (1999).

26. Kobayashi K, Ishii S, Kume K, Takahashi T, Shimizu T, Manabe T. Platelet-activating factor receptor is not required for long-term potentiation in the hippocampal CA1 region. Eur J Neurosci 11, 1313-6. (1999).

27. Hirabayashi T, Kume K, Hirose K, Yokomizo T, Iino M, Itoh H, Shimizu T. Critical duration of intracellular Ca2+ response required for continuous translocation and activation of cytosolic phospholipase A2. J Biol Chem 274, 5163-9. (1999).

28. Kume K, Zylka MJ, Sriram S, Shearman LP, Weaver DR, Jin X, Maywood ES, Hastings MH, Reppert SM. mCRY1 and mCRY2 are essential components of the negative limb of the circadian clock feedback loop. (COVER). Cell 98, 193-205. (1999).

29. Sato S, Kume K, Ito C, Ishii S, Shimizu T. Accelerated proliferation of epidermal keratinocytes by the transgenic expression of the platelet-activating factor receptor. Arch Dermatol Res 291, 614-21. (1999).

30. Nagase T, Uozumi N, Ishii S, Kume K, Izumi T, Ouchi Y, Shimizu T. Acute lung injury by sepsis and acid aspiration: a key role for cytosolic phospholipase A2. Nat Immunol 1, 42-6. (2000).

31. Tabuchi S, Kume K, Aihara M, Shimizu T. Expression of lysophosphatidic acid receptor in rat astrocytes: mitogenic effect and expression of neurotrophic genes. Neurochem Res 25, 573-82. (2000).

32. Kawasawa Y, Kume K, Nakade S, Haga H, Izumi T, Shimizu T. Brain-specific expression of novel G-protein-coupled receptors, with homologies to Xenopus PSP24 and human GPR45. Biochem Biophys Res Commun 276, 952-6. (2000).

33. Kawasawa Y, Kume K, Izumi T, Shimizu T. Mammalian PSP24s (alpha and beta isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems. Biochem Biophys Res Commun 276, 957-64. (2000).

34. Aihara M, Ishii S, Kume K, Shimizu T. Interaction between neurone and microglia mediated by platelet-activating factor. Genes Cells 5, 397-406. (2000).

35. Nakatani N, Uozumi N, Kume K, Murakami M, Kudo I, Shimizu T. Role of cytosolic phospholipase A2 in the production of lipid mediators and histamine release in mouse bone-marrow-derived mast cells. Biochem J 352 Pt 2, 311-7. (2000).

36. Shearman LP, Sriram S, Weaver DR, Maywood ES, Chaves I, Zheng B, Kume K, Lee CC, van der Horst GT, Hastings MH, et al. Interacting molecular loops in the mammalian circadian clock. (COVER). Science 288, 1013-9. (2000).

37. Yamashita A, Kawagishi N, Miyashita T, Nagatsuka T, Sugiura T, Kume K, Shimizu T, Waku K. ATP-independent fatty acyl-coenzyme A synthesis from phospholipid: coenzyme A-dependent transacylation activity toward lysophosphatidic acid catalyzed by acyl-coenzyme A:lysophosphatidic acid acyltransferase. J Biol Chem 276, 26745-52. (2001).

38. Morales J, Hiesinger PR, Schroeder AJ, Kume K, Verstreken P, Jackson FR, Nelson DL, Hassan BA. Drosophila fragile X protein, DFXR, regulates neuronal morphology and function in the brain. Neuron 34, 961-72. (2002).

39. Hendricks JC, Lu S, Kume K, Yin JCP, Yang Z, Sehgal A. Gender dimorphism in the role of cycle (BMAL1) in rest, rest regulation, and longevity in Drosophila melanogaster. J Biol Rhythms 18, 12-25. (2003).

40. Hegen M, Sun L, Uozumi N, Kume K, Goad ME, Nickerson-Nutter CL, Shimizu T, Clark JD. Cytosolic phospholipase A2alpha-deficient mice are resistant to collagen-induced arthritis. J Exp Med 197, 1297-302. (2003).

41. Kodama S, Toyonaga T, Kondo T, Matsumoto K, Tsuruzoe K, Kawashima J, Goto H, Kume K, Kume S, Sakakida M, et al. Enhanced expression of PDX-1 and Ngn3 by exendin-4 during beta cell regeneration in STZ-treated mice. Biochem Biophys Res Commun 327, 1170-8. (2005).

42. Kume K, Kume S, Park SK, Hirsh J, Jackson FR. Dopamine is a regulator of arousal in the fruit fly. J Neurosci 25, 7377-84. (2005).

43. Yoshida T, Shiraki N, Baba H, Goto M, Fujiwara S, Kume K, Kume S. Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas. Genes Cells 13, 667-78. (2008).

44. Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells 26, 874-85. (2008).

45. Shiraki N, Umeda K, Sakashita N, Takeya M, Kume K, Kume S. Differentiation of mouse and human embryonic stem cells into hepatic lineages. Genes Cells 13, 731-46. (2008).

46. AIZAWA R, SUNAHARA H, KUME K, TSUCHIYA S, ADACHI H, KANBAYASHI T, SHIMIZU T. Status of narcolepsy-related information available on the Internet in Japan and its effective use. Sleep Biol Rhythm 6, 201-207. (2008).

47. Shiraki N, Higuchi Y, Harada S, Umeda K, Isagawa T, Aburatani H, Kume K, Kume S. Differentiation and characterization of embryonic stem cells into three germ layers. Biochem Biophys Res Commun381, 694-9. (2009).

48. Yoshida T, Murata K, Shiraki N, Kume K, Kume S. Analysis of gene expressions of embryonic stem-derived Pdx1-expressing cells: implications of genes involved in pancreas differentiation. Dev Growth Differ 51, 463-72. (2009).

49. Matsuura K, Katsumoto K, Fukuda K, Kume K, Kume S. Conserved origin of the ventral pancreas in chicken. Mech Dev 126, 817-27. (2009).

50. Higuchi Y, Shiraki N, Yamane K, Qin Z, Mochitate K, Araki K, Senokuchi T, Yamagata K, Hara M, Kume K, et al. Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages. J Cell Sci 123, 2733-42. (2010).

51. Shiraki N, Harada S, Ogaki S, Kume K, Kume S. Identification of DAF1/CD55, a novel definitive endoderm marker. Cell Struct Funct 35, 73-80. (2010).

52. Matsuo A, Yoshida T, Yasukawa T, Miki R, Kume K, Kume S. Epiplakin1 is expressed in the cholangiocyte lineage cells in normal liver and adult progenitor cells in injured liver. Gene Expr Patterns 11, 255-62. (2011).

53. Ogaki S, Harada S, Shiraki N, Kume K, Kume S. An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells. BMC Dev Biol 11, 13. (2011).

54. Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Ozaki M, Hiraguchi T, Maeda T, Shiotani H, Terajima S, Sano H, Mori K, Yoshida M, Miyazato M, Kato J, Murakami N, Kangawa K, Kojima M. Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila. Biochem Biophys Res Commun 410, 872-7. (2011).

55. Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Yoshizawa-Kumagaye K, Nishio H, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M. Identification of the endogenous cysteine-rich peptide trissin, a ligand for an orphan G protein-coupled receptor in Drosophila. Biochem Biophys Res Commun 414, 44-8. (2011).

56. Shiraki N, Yamazoe T, Qin Z, Ohgomori K, Mochitate K, Kume K, Kume S. Efficient differentiation of embryonic stem cells into hepatic cells in vitro using a feeder-free basement membrane substratum. PLoS One 6, e24228. (2011).

57. Riemensperger T, Isabel G, Coulom H, Neuser K, Seugnet L, Kume K, Iché-Torres M, Cassar M, Strauss R, Preat T, et al. Behavioral consequences of dopamine deficiency in the Drosophila central nervous system. Proc Natl Acad Sci U S A 108, 834-9. (2011).

58. Nakai Y, Horiuchi J, Tsuda M, Takeo S, Akahori S, Matsuo T, Kume K, Aigaki T. Calcineurin and Its Regulator Sra/DSCR1 Are Essential for Sleep in Drosophila. J Neurosci 31, 12759-66. (2011).

59. Tomita J, Mitsuyoshi M, Ueno T, Aso Y, Tanimoto H, Nakai Y, Aigaki T, Kume S, Kume K. Pan-Neuronal Knockdown of Calcineurin Reduces Sleep in the Fruit Fly, Drosophila Melanogaster. J Neurosci 31, 13137-46. (2011).

60. Takahama K, Tomita J, Ueno T, Yamazaki M, Kume S, Kume K. Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila. Biochem Biophys Res Communic 417, 807-11. (2012).

61. Yamazaki M, Tomita J, Takahama K, Ueno T, Mitsuyoshi M, Sakamoto E, Kume S, Kume, K. High calorie diet augments age-associated sleep impairment in Drosophila. Biochem Biophys Res Communic 417, 812-6. (2012).

62. Ueno T, Fukuhara A, Ikegami A, Ohishi F, Kume K*. Monozygotic twins concordant for Kleine-Levin syndrome. BMC Neurol 12, 31. (2012).

63. Ida T, Takahashi T, Tominaga H, Sato T, Sano H, Kume K, Ozaki M, Hiraguchi T, Shiotani H, Terajima S, Nakamura Y, Mori K, Yoshida M, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M. Isolation of the bioactive peptides CCHamide-1 and CCHamide-2 from Drosophila and their putative role in appetite regulation as ligands for G protein-coupled receptors. Front Endocrinol 3, 177. (2012).

64. Ueno T, Tomita J, Kume S, Kume K*. Dopamine modulates metabolic rate and temperature sensitivity in Drosophila melanogaster. PLoS One 7, e31513. (2012).

65. Ueno T, Masuda N, Kume S, Kume K. Dopamine modulates the rest period length without perturbation of its power law distribution in Drosophila melanogaster. PLoS One 7, e32007. (2012).

66. Ueno T, Tomita J, Tanimoto H, Endo K, Ito K, Kume S, Kume K*. Identification of a dopamine pathway that regulates sleep and arousal in Drosophila. Nat Neurosci 15, 1516-1523. (2012).

67. Miki R, Yoshida T, Murata K, Oki S, Kume K, Kume S. Fate maps of ventral and dorsal pancreatic progenitor cells in early somite stage mouse embryos. Mech Dev 128, 597-609. (2012).

68. Umeda K, Suzuki K, Yamazoe T, Shiraki N, Higuchi Y, Tokieda K, Kume K, Mitani K, Kume S. Albumin gene targeting in human embryonic stem cells and induced pluripotent stem cells with helper-dependent adenoviral vector to monitor hepatic differentiation. Stem Cell Res 10, 179-194. (2012).

69. Ogaki S, Shiraki N, Kume K, Kume S. Wnt and Notch Signals Guide Embryonic Stem Cell Differentiation Into the Intestinal Lineages. Stem Cells 31, 1086-96. (2013).

70. Kataoka M, Kawamuro Y, Shiraki N, Miki R, Sakano D, Yoshida T, Yasukawa T, Kume K, Kume S. Recovery from diabetes in neonatal mice after a low-dose streptozotocin treatment. Biochem Biophys Res Commun 430, 1103-8. (2013).

71. Kawabata M, Ueno T, Tomita J, Kawatani J, Tomoda A, Kume S, Kume K*. Temporal organization of rest defined by actigraphy data in healthy and childhood chronic fatigue syndrome children. BMC Psych 13, 281. (2013).

72. Yamazoe T, Shiraki N, Toyoda M, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Umezawa A, Sasaki Y, Kume K, Kume, S. A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells. J Cell Sci 126, 5391-9. (2013).

73. Iwashita H, Shiraki N, Sakano D, Ikegami T, Shiga M, Kume K, Kume S. Secreted cerberus1 as a marker for quantification of definitive endoderm differentiation of the pluripotent stem cells. PLoS One 8, e64291. (2013).

74. Ueno T, Kume K*: Functional characterization of dopamine transporter in vivo using Drosophila melanogaster behavioral assays. Front Behav Neurosci 8: 303. (2014).

75. Hashizaki M, Nakajima H, Tsutusmi M, Shiga T, Chiba S, Yagi T, Ojima Y, Ikegami A, Kawabata M, Kume K*. Accuracy validation of sleep measurements by a contactless biomotion sensor on subjects with suspected sleep apnea. Sleep Biol Rhythm 12 12: 106-15. (2014)

76. Sakano D, Shiraki N, Kikawa K, Yamazoe T, Kataoka M, Umeda K, Araki K, Mao D, Matsumoto S, Nakagata N, Andersson O, Stainier D, Endo F, Kume K, Uesugi M, Kume S. VMAT2 Identified as a Regulator of Late-Stage β-Cell Differentiation. Nat Chem Biol 10, 141-8. (2014).

77. Shiraki N, Shiraki Y, Tsuyama T, Obata F, Miura M, Nagae G, Aburatani H, Kume K, Endo F, Kume S. Methionine metabolism regulates maintenance and differentiation of human pluripotent stem cells. Cell Metab 19: 780-94. (2014)

78. Kikawa K, Sakano D, Shiraki N, Tsuyama T, Kume K, Endo F, Kume S: Beneficial effect of insulin treatment on islet transplantation outcomes in Akita mice. PLoS One 9, e95451. (2014)

79. Shahjalal HM, Shiraki N, Sakano D, Kikawa K, Ogaki S, Baba H, Kume K, Kume S: Generation of insulin-producing β-like cells from human iPS cells in a defined and completely xeno-free culture system. J Mol Cell Biol 6: 394-408. (2014)

80. Tomita J, Ueno T, Mitsuyoshi M, Kume S, Kume K*: The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster. PLoS One 10: e0128101. (2015)

81. Hashizaki M, Nakajima H, Kume K*: Monitoring of Weekly Sleep Pattern Variations at Home with a Contactless Biomotion Sensor. Sensors (Basel) 15: 18950-64 (2015)

82. Sano H, Nakamura A, Texada MJ, Truman JW, Ishimoto H, Kamikouchi A, Nibu Y, Kume K, Ida T, Kojima M. The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster. PLoS Genet 11: e1005209. (2015)

83. Miyagawa T, Toyoda H, Kanbayashi T et al.: An association analysis of HLA-DQB1 with narcolepsy without cataplexy and idiopathic hypersomnia with/without long sleep time in a Japanese population. Hum Genome Var 2, 15031. (2015)

84. Toyoda H, Miyagawa T, Koike A, et al.: A polymorphism in CCR1/CCR3 is associated with narcolepsy. Brain Behav Immun 49, 148-55. (2015)

85. Miyagawa T, Toyoda H, Hirataka A et al.: New susceptibility variants to narcolepsy identified in HLA class II region. Hum Mol Genet 24, 891-8. (2015)

86. Yamamoto S, Ono H, Kume K, Ohsawa M. Oxaliplatin treatment changes the function of sensory nerves in rats. J Pharmacol Sci. 130, 189-93.(2016)

87. Nakashima R, Morooka M, Shiraki N, Sakano D, Ogaki S, Kume K, Kume S. Neural cells play an inhibitory role in pancreatic differentiation of pluripotent stem cells. Genes Cells 20, 1028-45 (2015)

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- [Review Articles]

1. Mori M, Bito H, Sakanaka C, Honda Z, Kume K, Izumi T, Shimizu T. Activation of mitogen-activated protein kinase and arachidonate release via two G protein-coupled receptors expressed in the rat hippocampus. Ann N Y Acad Sci 744, 107-25. (1994).*

2. Yokomizo T, Uozumi N, Takahashi T, Kume K, Izumi T, Shimizu T. Leukotriene A4 hydrolase and leukotriene B4 metabolism. J Lipid Mediat Cell Signal 12, 321-32. (1995).*

3. Shimizu T, Mori M, Bito H, Sakanaka C, Tabuchi S, Aihara M, Kume K. Platelet-activating factor and somatostatin activate mitogen-activated protein kinase (MAP kinase) and arachidonate release. J Lipid Mediat Cell Signal 14, 103-8. (1996).*

4.Jackson FR, Schroeder AJ, Roberts MA, McNeil GP, Kume K, Akten B. Cellular and molecular mechanisms of circadian control in insects. J Insect Physiol 47, 833-842. (2001).*

5. Kume K. A Drosophila dopamine transporter mutant, fumin (fmn), is defective in arousal regulation. Sleep and Biological Rhythms 4, 263-273. (2006).*